SnapShot: Mitochondrial Nucleoid

SnapShot: Mitochondrial Dynamics

SnapShot: Mitochondrial Architecture

Mammals C. elegans 1 TM domain; C-term in IMS and N-term in the matrix. IBM & CJ; self-oligomerizes, binds MICOS/MINOS/MitOS components and Sam50, Tob38, Ugo1, Fzo1. Yeast are viable but exhibit altered mtDNA inheritance and decreased respiratory growth. Mammals and worms exhibit increased ROS production and apoptosis. Loss of CJ, altered cristae structure, accumulation of cristae stacks and cr...

SnapShot: Mitochondrial Quality Control

Mitochondrial nucleoid interacting proteins support mitochondrial protein synthesis

Mitochondrial ribosomes and translation factors co-purify with mitochondrial nucleoids of human cells, based on affinity protein purification of tagged mitochondrial DNA binding proteins. Among the most frequently identified proteins were ATAD3 and prohibitin, which have been identified previously as nucleoid components, using a variety of methods. Both proteins are demonstrated to be required ...

Mitochondrial Nucleoid: Shield and Switch of the Mitochondrial Genome

Mitochondria preserve very complex and distinctively unique machinery to maintain and express the content of mitochondrial DNA (mtDNA). Similar to chromosomes, mtDNA is packaged into discrete mtDNA-protein complexes referred to as a nucleoid. In addition to its role as a mtDNA shield, over 50 nucleoid-associated proteins play roles in mtDNA maintenance and gene expression through either tempora...